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Friday, 06 November, 2009

A nanoscopic ruler made of DNA

NIM researchers from the groups of Friedrich Simmel and Philip Tinnefeld at TU and LMU Munich created a nanoscopic ruler made of DNA.

The improvement of optical microscopy towards ever higher resolution has been subject to extensive research over the past years. The diffraction limit of light prohibits resolving details smaller than half its wavelength, resulting in a fundamental resolution limit of 250-300 nm. It is the goal, however, to reach a resolution that enables imaging of much smaller, closely packed structures by optical microscopy. Exciting developments in fluorescence microscopy have enabled far-field imaging beyond this diffraction limit. Several approaches have been realized within the last years yielding fluorescence images of unprecedented sharpness and resolution. One such method that uses the subsequent localization of single fluorescent molecules has been developed by the group of Prof. Tinnefeld at the LMU (Blink-Microscopy, see press release May 12, 2009).

For further development of the field, structures with defined numbers of fluorescent molecules at precisely determined positions are required that allow comparison and evaluation of the approaches as well as calibration of each superresolution fluorescence microscope. In this context, a nano-scale ruler would be very much appreciated.

Independent of the developments of optical microscopy, physicists from the group of Prof. Simmel at the Technical University of Munich have advanced the use of self-assembled DNA nanostructures. In the so-called "DNA origami technique", single stranded DNA molecules can be made to self-assemble into well-defined two-dimensional macromolecular structures. The name "origami" comes from the fact that a long single stranded DNA molecule is "folded" into a particular shape. With a diameter of typically 100 nm and a fully addressable breadboard-like structure, rectangular DNA origami structures can be used to arrange nanoscale objects with nanometer precision. This very feature makes DNA origami the ideal sample for super-resolution microscopy.

Brought together by NIM and the Center for Nanoscience, researchers from TU and LMU Munich shared their expertise to create a nanoscopic ruler made of DNA. They showed that different super-resolution techniques can be used to resolve the distance between two fluorophores grafted into the DNA origami structure. The theoretically designed distance was not only measured experimentally with a deviation of only one nanometer, but it could also be shown that blink microscopy is capable of optically resolving 90 % of the structures. This demonstrates the robustness of both DNA origami as nanoscopic ruler as well as blink microscopy, thus offering the required calibration structures for superresolution microscopies.

Original Publication:
[1] C. Steinhauer, R. Jungmann, T. L. Sobey, F. C. Simmel, P. Tinnefeld, DNA Origami as Nanoscopic Ruler for Superresolution Microscopy, Angew Chem Int Ed 2009, Early View

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