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NIM nanosystems initiative munich

Thursday, 18 July, 2013

Cell Sorting and Directed Evolution in Microfluidic Systems

Special Seminar Augsburg University

Speaker: Prof. H. Tom Soh, Departments of Materials, Chemical and Mechanical Engineering, University of California – Santa Barbara

Time: Thursday, 18 July 2013, 15:00h

Venue: Augsburg University, Universitätsstraße 1, Room T2003 (Hörsaalzentrum Physik)


Current techniques in high performance molecular and cellular separations are limited by the inherent coupling among three competing parameters: throughput, purity, and recovery of rare species.

Our group utilizes unique advantages of microfluidics technology to decouple these competing parameters by precise and reproducible generation of separation forces that are not accessible in conventional, macroscopic systems.

In this seminar, we will first discuss novel high performance electrokinetic, magnetophoretic and acoustophoretic separation systems to purify rare target cells from complex mixtures. Next, we will present our recent work in applying the microfluidic separation systems for Rapid Directed Evolution of molecules (RDE). We will provide theoretical and experimental evidence for extremely fast generation of affinity reagents –molecular recognition elements that bind to target molecules with high affinity and specificity.

Finally, we will present innovative methods of evolving molecular machines that are capable of performing complex functions, including binding induced switching and conformation change.


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